FORMATE-PYRUVATE EXCHANGE REACTION IN STREPTOCOCCUS FAECALIS I
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چکیده
منابع مشابه
Formate-pyruvate Exchange Reaction in Streptococcus Faecalis. I. Factor Requirement for Intact Cells.
Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyruvate by Streptococcus faecalis 10Cl. Yeast extract produced a response linear...
متن کاملThe pyruvate formate-lyase system of Streptococcus faecalis. I. Purification and properties of the formate-pyruvate exchange enzyme.
The enzyme exchanging formate with the carboxyl of pyruvate was purified 195-fold from extracts of Streptococcus fuecalis by a procedure involving protamine treatment, dialysis, and gradient centrifugation. Centrifugation of protamine-treated extract in a 10 to 40% potassium tartrate gradient provided a 65-fold purhication and separated the exchange enzyme from pyruvate decarboxylase, acetolact...
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In the over-all reaction, one molecule of diacetyl is oxidized to two molecules of acetate, and another molecule of diacetyl is reduced to acetoin. The reaction was shown to require cocarboxylase (DPT).2 Subsequently, Schweet et al. (1951) expanded this finding and pointed out the similarity between the diacetyl reaction and pyruvic acid oxidation, with respect to the metabolism of the "active ...
متن کاملFormate fixation in pyruvate by Escherichia coli.
The phosphoroclastic decomposition of pyruvic acid to acetic and formic acids by enzyme extracts of Escherichia coli has been an object of study ever since acetyl phosphate was tentatively identified as an intermediate of this reaction (1). The finding by Utter, Lipmann, and Werkman that isotopic carbon of formate was incorporated into the carboxyl group and that the carboxyl carbon of acetate ...
متن کاملOxygen sensitivity of sugar metabolism and interconversion of pyruvate formate-lyase in intact cells of Streptococcus mutans and Streptococcus sanguis.
Pyruvate formate-lyase (PFL) (formate acetyltransferase; EC 2.3.1.54) of oral streptococci is essential for metabolizing sugar into volatile compounds (formate, acetate, and ethanol). This enzyme is extremely sensitive to oxygen, and its activity is irreversibly inactivated by oxygen. When Streptococcus sanguis was anaerobically starved, a part of the active form of PFL was converted into a rev...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1964
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.87.1.97-103.1964